High school9 min

Diluting a solution: dilution factor and protocol

The one idea to hold on to — the amount of solute does not change —, working out the volume to pipette, the right glassware and serial dilutions.

The essentials

1

What is conserved

Diluting means adding solvent: the amount of solute does not change. C1×V1=C2×V2C_1 \times V_1 = C_2 \times V_2 C1C_1 and V1V_1: concentration of the stock solution and volume taken from it. C2C_2 and V2V_2: concentration and total volume of the working (diluted) solution.
2

The dilution factor

Members only

3

The glassware

Members only

4

Serial dilutions

Members only

5

Checking with absorbance

Members only

ƒWrite my own sheetOr start from a blank page: rewriting a concept in your own words is how it sticks.